Preliminary Qualitative Phytochemical Screening and in Vitro Hypoglycemic Potential of Acanthus Ilicifolius and Evolvulus Emerginatus

Objective: The present study was planned to explore phytoconstituents and hypoglycemic potential of the leaf extracts of Acanthus ilicifolius and Evolvulus emerginatus by invitro method. Methods: The aqueous, ethanol and methanol leaf extracts were prepared by soxhlation method. Qualitative phytochemical screening was done by using Horborne method. Methanol leaf extracts and standard drug acarbose were analyzed for their inhibition on α-amylase and α-glucosidase by standard DNSA method. Glucose diffusion inhibitory activity was analyzed by using dialysis membrane. IC50 Results: Phytochemical analysis revealed that the methanol leaf extract contains maximum phytoconstituents than aqueous and ethanol extract. Methanol leaf extracts showed maximum inhibition on α-amylase and α-glucosidase inhibitory effect in a concentration dependant manner. Inhibitory activity of A. ilicifolius on α-amylase was 82.32 ± 0.02% (IC values were calculated from the dose response curve of standard drug acarbose. 50 33.13 ± 0.030μg /ml) and on α-glucosidase was 79.35 ± 0.02% (IC50 39.42 ± 0.01μg/ml). E.emerginatus extract inhibitory effect on αamylase was 78.26 ± 0.014% (IC50 29.76 ± 0.07μg/ml) and on α-glucosidase was 80.8 ± 0.08% (IC50 28.04 ± 0.01μg/ml).The inhibitory activity of acarbose on α-amylase was 84.11 ± 0.57% (IC50 44.16 ± 0.15μg/ml) and on α-glucosidase was 85.0 ± 0.22% (IC50 Conclusion: The invitro analysis proves the function of extracts in lowering glucose absorption and decrease post prandial blood glucose concentration. Hence these plant extracts can be used for invivo animal studies and development of a new drug without any harmful side effects. 39.86 ± 0.34μg /ml). Methanol leaf extracts of these two plants effectively retarded the glucose diffusion across the dialysis membrane.